Jiefei Wang Welcome! I am a Ph.D. student at Indiana University Bloomington, where I work with my advisor Prof. Dr. David P. Giedroc on Transition Metal Homeostasis in Bacterial Pathogens. I received my B.S. in Biosciences, University of Science and Technology of China in 2013. My research interests lie in quantitative chemical biology of infectious disease, using massive amount of data associated with -omics approaches, biochemistry and structure determination. Contact: Simon Hall 310, Department of Molecular and Cellular Biochemistry, Indiana University Bloomington, Bloomington, IN 47405 Email: jiefwang [AT: indiana.edu] Curriculum Vitae ResearchGate LinkedIn

Education

• September 2013 – present

• Ph.D candidate, Biochemistry, Indiana University Bloomington

• Advisor: David P. Giedroc
• September 2009 – May 2013

• B.S., Biosciences, University of Science and Technology of China

• Advisor: Changlin Tian

Research Experience

• Graduate Research Assistant, Jan. 2014 – now

• Project: The response of Acinetobacter baumannii to human-mediated metal starvation

• Discovered response to calprotectin-induced iron and zinc starvation in A. baumannii via metal quantification with ICP-MS, transcriptome and proteome analysis; biochemically and structurally characterized a new player involved in the dual regulation of flavin biosynthesis pathway.

• Discovered a putative Zn-dependent cell wall-modifying enzyme via UPLC-MS-based peptidoglycan profiling.

• Discovered the role of histidine in Zn-buffering in maintaining Zn homeostasis through characterizing metal binding and enzymetic acitivity of a putative GTPase-dependent Zn chaperone and its potential client target in histidine utilization pathway.




• Research Assistant, July. 2012 – May. 2013

• Project: Site-specific incorporation of unnatural amino acid at two sites and distance measurement by ¹⁹F NMR

• Inspected protein-protein interaction by ¹⁹F NMR.

Publications

• Wang, J.*, Lonergan, Z. R.*, Gonzalez-Guitierrez, G., Nairn, B. L., Maxwell, C. N., Zhang, Y., Andreini, C., Karty, J. A., Chazin, W. J., Trinidad, J. C., Skaar, E. P., and Giedroc, D. P. (2019) Multi-metal restriction by calprotectin impacts de novo flavin biosynthesis in Acinetobacter baumannii. Cell Chem Biol, in the press




• Lonergan, Z. R., Nairn, B. L., Wang, J., Hsu, Y. P., Hesse, L. E., Beavers, W. N., Chazin, W. J., Trinidad, J. C., VanNieuwenhze, M. S., Giedroc, D. P., and Skaar, E. P. (2019) An Acinetobacter baumannii, zinc-regulated peptidase maintains cell wall integrity during immune-mediated nutrient sequestration. Cell Rep 26, 2009-2018. [Link]




• Capdevila, D. A., Wang, J.,and Giedroc, D. P. (2016) Bacterial strategies to maintain zinc metallostasis at the host-pathogen interface. J Biol Chem 291, 20858-20868. [Link]




• Nairn, B. L., Lonergan, Z. R., Wang, J., Braymer, J. J., Zhang, Y., Calcutt, M. W., Lisher, J. P., Gilston, B. A., Chazin, W. J., de Crecy-Lagard, V., Giedroc, D. P., and Skaar, E. P. (2016) The response of Acinetobacter baumannii to zinc starvation. Cell Host & Microbe 19, 826-836. [Link]

Manuscript

• Wang, J.*, Capdevila, D. A.*, and Giedroc, D. P. (2019) Metal ion homeostasis. Invitated chapter for Comprehensive Coordination Chemistry III (Editors: Yi Lu & Lawrence Que Jr.), Elsevier

Teaching

• Laboratory instructor, C127 Principles of Chemistry and Biochemistry I, 2014 Spring, Summer & Fall
  

• Lectured, instructed and trained students on scientific principles common to all science disciplines and to teach specific laboratory skills and software important in the study of chemistry and biochemistry.
  

• Graded assignments and exams.
  

• Laboratory instructor, C487 Biochemistry (upper level undergraduate course), 2015 Spring
  

• Lectured, instructed and trained students on fundamental laboratory approaches for biochemistry and biotechnology.
  

• Graded assignments and exams.
  

Activities

• Oral Presentations
  

• March 2019, BMB series seminar, Department of Molecular and Cellular Biochemistry, Indiana University
  

• July 2016, Gordon Research Seminar (GRS) - Cell Biology of Metals, West Dover, VT
  

• March 2016, BMB series seminar, Department of Molecular and Cellular Biochemistry, Indiana University
  

• Poster Presentations
  

  • June 2018, Summer Symposium in Molecular Biology, State College, PA
    

  • July 2016, Gordon Research Conference (GRC) - Cell Biology of Metals, West Dover, VT